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Saturday, April 18, 2020 | History

2 edition of Effect of lipopolysaccharide on monocyte cytokine secretion transduced by mek and erk found in the catalog.

Effect of lipopolysaccharide on monocyte cytokine secretion transduced by mek and erk

Marisa Tessier

Effect of lipopolysaccharide on monocyte cytokine secretion transduced by mek and erk

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  • 34 Currently reading

Published by Laurentian University in Sudbury, Ont .
Written in English


Edition Notes

Statementby Marisa Tessier, Fahamia Koudra
ContributionsKoudra, Fahamia.
The Physical Object
Paginationiv, 29 l. :
Number of Pages29
ID Numbers
Open LibraryOL22154862M

Lipopolysaccharide (LPS) binding protein opsonizes LPS-bearing particles for recognition by a novel receptor on macrophages. Wright S.D., Tobias P.S., Ulevitch R.J., Ramos R.A. Lipopolysaccharide binding protein (LBP) is an acute-phase reactant that binds bacterial LPS.   Pyrogen detection is of utmost importance in pharmaceutical industry, laboratories and health care institutions. As an alternative to the animal-consuming rabbit pyrogen test or Limulus amoebocyte lysate test, the monocyte activation test was introduced as a gold standard method in the European Pharmacopoeia. However, the monocyte activation test has not gained wide acceptance in Cited by: Marantos, Christos, Mukaro, Violet, Ferrante, Judith, Hii, Charles and Ferrante, Antonio , Inhibition of the lipopolysaccharide-induced stimulation of the members of the MAPK family in human monocytes/macrophages by 4-hydroxynonenal, a product of oxidized omega-6 fatty acids, American journal of pathology, vol. , no. 4, pp. T1 - Lipopolysaccharide-Binding Protein Critically Regulates Lipopolysaccharide-Induced IFN-β Signaling Pathway in Human Monocytes. AU - Kato, Atsushi. AU - Ogasawara, Takahisa. AU - Homma, Toshiki. AU - Saito, Hirohisa. AU - Matsumoto, Kenji. PY - /5/ Y1 - /5/15Cited by:


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Effect of lipopolysaccharide on monocyte cytokine secretion transduced by mek and erk by Marisa Tessier Download PDF EPUB FB2

Pertussis toxin (inhibitor of G αi protein-receptor coupling) has been shown to block monocyte transmigration induced by the cytokine resistin by inhibiting fractalkine and MCP1 secretion.

18,25 Moreover, gallein (inhibitor of G βγ subunit-dependent signaling) suppressed macrophage chemotaxis and inhibited the activation of p38, JNK, and ERK Cited by: 2.

Lipopolysaccharide activation of the MEK-ERK1/2 pathway in human monocytic cells mediates tissue factor and tumor necrosis factor alpha expression by inducing Elk-1 phosphorylation and Egr-1 expression.

Guha M(1), O'Connell MA, Pawlinski Cited by:   Lipopolysaccharide (LPS) 1, an outer membrane component of Gram negative bacteria, is a potent activator of monocytes and triggers the abundant secretion of many cytokines from macrophages including IL-1 (), IL-6 (), and TNF-α (), which together contributes to the pathophysiology of septic major cell surface receptor for LPS on macrophages is CD14, a Cited by: Effect of cytokines and lipopolysaccharide on CD14 antigen expression in human monocytes and macrophages.

Authors. Landmann, Corresponding author. Department of Research, University Hospital, Basel, Switzerland. Lipopolysaccharide-induced inflammation in monocytes/macrophages is blocked by liposomal delivery of Gi-protein inhibitor Monica Madalina Tucureanu,1,* Daniela Rebleanu,1,* Cristina Ana Constantinescu,1,2 Mariana Deleanu,3,4 Geanina Voicu,1 Elena Butoi,1 Manuela Calin,1 Ileana Manduteanu1 1Department of Biopathology and Therapy of Inflammation, Nicolae Simionescu Cited by: 2.

The maximal activity of LPS-induced ERK, JNK, and p38 was (30 min), 5- (30 min), and fold (15 min), respectively. In contrast, NAC reduced ERK activity to 60% and decreased p38 activity to the basal level, but JNK activity was induced 2-fold. The first experiment examined the effect of inhibiting two different MAPK (ERK and p38) pathways involved in the innate response to bacterial LPS on production of IL This experiment demonstrated that expression of the IL-6 gene is mediated by two of these MAPK signaling pathways (i.e.

ERK and p38).Cited by:   In vitro cell culture models are widely used in inflammation research; however, information regarding the time- and dose-dependency of inflammatory responses toward LPS in these cell lines is scattered in the literature.

JA.1 mouse macrophage and THP-1 human monocyte cell lines. JA.1 and THP-1 cells were treated with 0– ng/mL lipopolysaccharide for 0–24 by:   Lipopolysaccharide (LPS 1 or endotoxin) is a potent activator in the immune system.

It induces a variety of immune responses to severe infection (), such as local inflammation, antibody production, and septic addition, LPS is the major mediator of the high morbidity and mortality rates characteristic of Gram-negative bacteria, i.e. endotoxic shock (3, 5). Our ex-vivo impaired monocyte model aimed to determine the effect IκK has on monocyte function in reference to the well-known comparators IFN-γ and GM-CSF.

IFN-γ is a pleiotropic cytokine shown to reverse monocyte impairment in-vitro and in-vivo and polarizes macrophages to an M1-like phenotype (Leentjens et al.,Mosser, Cited by: 2.

Characterization of lipopolysaccharide-stimulated cytokine expression in macrophages and monocytes Article in Agents and Actions 61(12) July with 45 Reads How we measure 'reads'. Sodium Lactate and Lipopolysaccharide Exert Synergistic Effect on MMP and Cytokine Expression in U Histiocytes through NFkB and MAPK Pathways The plasma lactate concentrati The plasma lactate concentration in patients with obesity and type 2 diabetes is often higher than that in.

Monocytes can be primed in vitro by lipopolysaccharide (LPS) for release of cytokines, for enhanced killing of cancer cells, and for enhanced release of microbicidal oxygen radicals like superoxide and peroxide. We investigated the proteins involved in regulating priming, using 2D gel proteomics.

Monocytes from 4 normal donors were cultured for 16 h in chemically defined medium in Teflon bags Cited by:   Effect of durations of boiling on the cytokine‐inducing activity of LPS To assess the heat sensitivity of LPS cytokine‐inducing activity, investigators have heated LPS in a boiling water bath for various intervals ranging from 15 min to 60 min and tested the cytokine‐inducing activities of LPS at concentrations ranging from Cited by: Background: Lipopolysaccharide (LPS) is known to activate macrophages, causing the release of toxic cy-tokines that may provoke inflammation and shock.

One of the most important andbest studied of. / High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP In: Journal of Endotoxin Research. ; Cited by:   Thus, activation of the transcription factor NFκB is a critical step in monocyte inflammatory cytokine production after exposure to bacterial stimuli such as LPS (4–6).

Among the various NFκB/Rel dimers the p65/p50 heterodimer is the prototypical form of LPS-inducible NFκB in monocytes (7, 8).Cited by: Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood N.E.

Gomes1, M.K.C. Brunialti1, M.E. Mendes1, M. Freudenberg2, C. Galanos2 and R. Salomão1 1Disciplina de Infectologia, Universidade Federal de. Effects of LPS and AEBSF on cytokine secretion by monocytes.

Four of the ten cytokines assayed in culture medium from monocytes cultured for 16 h ± LPS ± AEBSF are shown.

The first bar is Time 0, a control of monocyte medium taken at the start of culture. The last two bars show the effect of AEBSF added at the end of the culture at 16 by:   The in vitro immune responsiveness of cells from germfree (GF) mice was investigated by employing lectins and endotoxin.

Splenocytes from GF, when compared with those from conventional (Conv), mice manifested similar responses to the T lymphocyte mitogens, PHA and Con by:   Prolactin from pituitary gland helps maintain homeostasis but it is also released in immune cells where its function is not completely understood.

Pleiotropic functions of prolactin (PRL) might be mediated by different isoforms of its receptor (PRLr). The aim of this study was to investigate the relationship between the eventual synthesis of PRL and PRLr isoforms with the inflammatory response Cited by:   Lipopolysaccharide induces monocyte chemoattractant protein production by rat mesangial cells.

The Journal of Laboratory and Clinical Medicine, (1), Lipopolysaccharide induces monocyte chemoattractant protein production by rat mesangial by:   Previous studies have shown that lipopolysaccharide (LPS) induces acute lung injury (ALI), and that endothelial progenitor cells (EPCs) participate in tissue repair.

Therefore, in this study it was hypothesized that LPS influences the number and function of EPCs directly. In order to investigate this, an in vitro study was performed using EPCs. the effect of NETs on cytokine production by macrophages, mouse macrophage‑like J cells were treated with NETs in combination with lipopolysaccharide (LPS) as a constituent of pathogen-associated molecular patterns.

The results revealed that NETs significantly induced the production of interleukin (IL)-1β by J cells in the presence of LPS. Systemic inflammation shifts the brain microenvironment towards a proinflammatory state.

However, how peripheral inflammation mediates changes in Cited by:   The p38α mitogen-activated protein kinase (MAPK) is a well-characterized intracellular kinase involved in the overproduction of proinflammatory cytokines from glia.

As such, p38α appears to be a promising therapeutic target for neurodegenerative diseases associated with neuroinflammation. However, the in vivo role of p38α in cytokine production in the CNS is poorly defined, and prior work Cited by: 6. immune cells that play a prominent role in the regulation of immune responses.

Cytokine mediated effects range from impacting immune cell migration to sites of injury, to regulating the inflammatory responses, and to determining the differentiation of immune cells.

A key player in the adaptive response is the CD4+ T helper (Th) cell. Jozefowski, S., Bobek, M. & Marcinkiewicz, J. Exogenous but not endogenous prostanoids regulate cytokine secretion from murine bone marrow dendritic cells: EP2, DP, and IP but not EP1, EP3, and FP Cited by: Wnt5a signaling has been implicated in the progression of cancer by regulating multiple cellular processes, largely migration and invasion, epithelial-mesenchymal transition (EMT), and metastasis.

Since Wnt5a signaling has also been involved in inflammatory processes in infectious and inflammatory diseases, we addressed the role of Wnt5a in regulating NF-κB, a pivotal mediator of Cited by: 1.

Immunity Article Lipopolysaccharide Induces Alveolar Macrophage Necrosis via CD14 and the P2X7 Receptor Leading to Interleukin-1a Release Jargalsaikhan Dagvadorj,1 Kenichi Shimada,1,2 Shuang Chen,1,2 Heather D. Jones,2,3 Gantsetseg Tumurkhuu,1 Wenxuan Zhang, 1Kolja A. Wawrowsky,4 Timothy R.

Crother,1,2 and Moshe Arditi,2 5 * 1Departments of Biomedical Sciences, Medicine and. The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages Ruairi C. Robertson 1,2, Freddy Guihéneuf 3, Bojlul Bahar 4, Matthias Schmid 3, Dagmar B.

Stengel 3, Gerald F. Fitzgerald 2,5, R. Paul Ross 5,6 and Catherine Stanton 1,5,*. Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

Kramecyne is a new peroxide, it was isolated from Krameria cytisoides, methanol extract, and this plant was mostly found in North and South America. This compound showed potent anti-inflammatory activity; however, the mechanisms by which this compound exerts its anti-inflammatory effect are not well understood.

In this study, we examined the effects of kramecyne on inflammatory responses in Cited by: Read "Lipopolysaccharide (LPS) down‐regulates CD4 expression in primary human macrophages through induction of endogenous tumour necrosis factor (TNF) and IL‐1β, Clinical & Experimental Immunology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

Flow Cytometric Measurement of Signal Transduction Pathways in Lipopolysaccharide (LPS) Activated Human Peripheral Blood Monocytes Abstract: LPS is known to activate multiple signaling pathways in peripheral blood monocytes via the TLR4 receptor complex.

Following LPS exposure in File Size: 43KB. T1 - Inhibition of interleukin production in lipopolysaccharide- activated mouse macrophages by hpyericin, an active component of Hypericin perforatum. AU - Kang, B.

AU - Chung, S. AU - Kim, Tae Sung. PY - /7/9. Y1 - /7/9Cited by: Mice were administered either vehicle controls or selective P2Y1, P2Y12, P2Y14, or P2X1 antagonists intravenously before intranasal administration of lipopolysaccharide (LPS) to investigate the effect of these drugs on pulmonary leukocyte recruitment, peripheral platelet counts, bleeding times, and ex vivo platelet by:   Macrophages sense microorganisms through activation of members of the Toll-like receptor family, which initiate signals linked to transcription of many inflammation associated genes.

In this paper we examine whether the signal from Toll-like receptors [TLRs] is sustained for as long as the ligand is present, and whether responses to different TLR agonists are by: Effect of lipopolysaccharide on B16F10 and 4T1 cell lines vi-ability The MTT assay was used to determine the effects of TLR4 stimulation on B16F10 and 4T1 cell proliferation.

The cells were exposed to culture medium or ultra-pure LPS (0, 5,10 μg/ml) for 4, 16, 24 and 48 h. " 분비나무 줄기 수피 추출물에 의한 HeLa Cell Line의 증식억제 효과와 RAW 세포에서 Lipopolysaccharide에 의해 유도된 Nitric Oxide 생성 저해효과 " " Inhibitory Effect on the Lipopolysaccharide-Induced Nitric Oxide Formation in RAW and on the Proliferation of HeLa Cell Lines by the Stem Bark Extracts.

Blockage of HO-1 expression using siRNA reversed the inhibitory effect of celastrol on HIV-1 Tat-induced inflammatory responses. These results suggest that celastrol has regulatory effects on HIV-1 Tat-induced inflammatory responses by blocking the JNK MAPK-AP-1/NF-κB signaling pathways and inducing HO-1 expression in astrocytes.Lipopolysaccharide (LPS) increases the invasive ability of pancreatic cancer cells through the TLR4/MyD88 signaling pathway Mio Ikebe, Yoshiki Kitaura, Masafumi Nakamura, Haruo Tanaka, Akio Yamasaki, Shuntaro Nagai, Junji Wada, Kosuke Yanai, Kenichiro Koga, Norihiro Sato, Makoto Kubo, Masao Tanaka, Hideya Onishi, Mitsuo KatanoCited by: products of lipopolysaccharide-activated macrophages (tumor necrosis factor-cy, transforming growth [email protected]) but not lipopolysaccharide modify dna synthesis by rat trophoblast cells exhibiting the kda lipopolysaccharide-binding protein' joan s.

hunt,'* michael j. soares,' mei-guey lei.* rex .